5UWP

Crystal Structure of mDia2 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.183, 106.183, 303.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.442 - 2.054
R-factor	0.1788
Rwork	0.178
R-free	0.21260
RMSD bond length	0.003
RMSD bond angle	0.547
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.079	0.957
Rpim	0.034	0.409
Number of reflections	108570	5319
<I/σ(I)>	23.2	1.9
Completeness [%]	99.9	100
Redundancy	6.4	6.5
CC(1/2)		0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 8 mM HCl