5UWA

Structure of E. coli phospholipid binding protein MlaC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	33.630, 115.890, 133.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.718 - 1.501
R-factor	0.1387
Rwork	0.138
R-free	0.16750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	based on homology model with 2QGU as a template
RMSD bond length	0.009
RMSD bond angle	1.202
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	83295
<I/σ(I)>	22.2
Completeness [%]	98.5
Redundancy	7.2
CC(1/2)	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M citric acid pH 3.5 and 1.6 M ammonium sulfate