5UW8

Structure of E. coli MCE protein MlaD, core MCE domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	C 1 2 1
Unit cell lengths	139.660, 103.820, 77.210
Unit cell angles	90.00, 111.03, 90.00

Refinement procedure

Resolution	42.120 - 2.150
R-factor	0.2053
Rwork	0.204
R-free	0.24360
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.939
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.150
Number of reflections	55590
<I/σ(I)>	15.4
Completeness [%]	99.3
Redundancy	7.6
CC(1/2)	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium acetate pH 4.5, and 40% 1,2 propanediol