5UVU

BRD4_BD2_A-1461028

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	63.712, 70.541, 33.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 1.660
R-factor	0.19
Rwork	0.189
R-free	0.21200
RMSD bond length	0.010
RMSD bond angle	0.890
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.300
High resolution limit [Å]	1.660
Rmerge	0.072
Number of reflections	18674
<I/σ(I)>	18.8
Completeness [%]	99.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 MM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol, Tris PH 7.0