5UVL

Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	P 43 21 2
Unit cell lengths	68.300, 68.300, 108.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.650
R-factor	0.22789
Rwork	0.225
R-free	0.24269
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5avj
RMSD bond length	0.009
RMSD bond angle	1.137
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	2.650
Number of reflections	6769
<I/σ(I)>	4.6
Completeness [%]	99.2
Redundancy	104.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	293	0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride