5UVI
Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-08-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.300, 180.300, 142.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.200 |
| R-factor | 0.24786 |
| Rwork | 0.241 |
| R-free | 0.28721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k2b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.614 |
| Data reduction software | CrystFEL (0.6.2) |
| Data scaling software | CrystFEL (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 7702 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.8 | 98.32 |
| Redundancy | 142.6 | |
| CC(1/2) | 0.992 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | 0.1 M sodium citrate pH 5.0, 32 % PEG 400, 75 mM sodium thocyanate |
