5UV6

Crystal structure of human Opioid Binding Protein/Cell Adhesion Molecule Like (OPCML)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.97931
Spacegroup name	P 41 21 2
Unit cell lengths	93.564, 93.564, 262.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.443 - 2.650
R-factor	0.284949700167
Rwork	0.284
R-free	0.30028
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.882
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.500	2.744
High resolution limit [Å]	2.650	2.650
Rmerge	0.170	2.740
Rpim	0.047	0.740
Number of reflections	34665	3401
<I/σ(I)>	9.71	1.2
Completeness [%]	95.8	88.8
Redundancy	14.4	14.5
CC(1/2)	0.996	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	A 50/50 (v/v) mix of 1.6 M MgSO4, 0.1 M MES pH 6.5 with 20% PEG 8000, 100 mM HEPES pH 7.5.