5UTC
Deoxy form of sperm whale myoglobin V68A/I107Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.454, 30.565, 63.811 |
| Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
| Resolution | 26.370 - 1.800 |
| R-factor | 0.1887 |
| Rwork | 0.186 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.875 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.000 | 31.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.046 | 0.044 | 0.070 |
| Rmeas | 0.056 | 0.054 | 0.089 |
| Rpim | 0.031 | 0.032 | 0.054 |
| Number of reflections | 12004 | ||
| <I/σ(I)> | 39.5 | ||
| Completeness [%] | 99.0 | 96.5 | 94.3 |
| Redundancy | 3 | 2.6 | 2.1 |
| CC(1/2) | 0.994 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M Tris-HCl, 1 mM EDTA, pH 7.4 2.56 M Ammonium Sulfate |
