5USO
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (GGTTACrGrGrU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.560, 57.610, 66.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.616 - 2.000 |
| R-factor | 0.2139 |
| Rwork | 0.210 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HI4K |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (0.5.15) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.760 | 66.760 | 1.980 |
| High resolution limit [Å] | 1.930 | 9.060 | 1.930 |
| Rmerge | 0.163 | 0.078 | 0.897 |
| Rmeas | 0.201 | 0.089 | 1.303 |
| Rpim | 0.089 | 0.041 | 0.656 |
| Total number of observations | 62798 | 697 | 2817 |
| Number of reflections | 13269 | ||
| <I/σ(I)> | 5.7 | 11.6 | 1.1 |
| Completeness [%] | 98.9 | 97.9 | 94.6 |
| Redundancy | 4.7 | 4.4 | 3.4 |
| CC(1/2) | 0.987 | 0.992 | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Tris, 0.2 mM sodium formate, 15% PEG 4000 |
