5USG
5-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.952, 37.827, 62.070 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 35.506 - 1.700 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.22128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8u |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Rpim | 0.023 |
| Number of reflections | 20662 |
| <I/σ(I)> | 31.5 |
| Completeness [%] | 100.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 12%(w/v) PEG 20000 |
