5UR3
Kaposi's Sarcoma Herpesvirus Protease in Complex with Allosteric Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.400, 92.860, 118.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.640 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.339 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHENIX (Phenix 1.8.4 Phaser 2.1) |
| Refinement software | PHENIX (1.10PRE_2100) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.470 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 37258 |
| <I/σ(I)> | 21.4 |
| Completeness [%] | 99.8 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.3M Imidazole pH 8.0, 0.4M NaH2PO4/1.6M K2HPO4, 0.2M NaCl |
