5UQC
Crystal structure of mouse CRMP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1271 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.412, 107.222, 81.680 |
| Unit cell angles | 90.00, 120.66, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.780 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gse |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 1.570 |
| High resolution limit [Å] | 1.780 | 1.540 |
| Rmerge | 0.059 | 0.539 |
| Rpim | 0.026 | |
| Number of reflections | 552156 | |
| <I/σ(I)> | 23.1 | |
| Completeness [%] | 99.9 | 82.8 |
| Redundancy | 5.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 0.1 M Bis-Tris pH 6, 25% PEG 3350 |
