5UOO
BRD4 bromodomain 2 in complex with CD161
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.188, 72.793, 32.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.380 - 1.690 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.380 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.690 | 4.610 | 1.700 |
| Rmerge | 0.071 | 0.046 | 0.418 |
| Number of reflections | 14010 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 97.9 | 98.6 | 87.1 |
| Redundancy | 6.1 | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 60% peg 400, 0.1M Imidizole, 5mM ATP |
