5UN7

Structure of the human POT1-TPP1 telomeric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 41 2 2
Unit cell lengths	99.530, 99.530, 122.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.18432
Rwork	0.182
R-free	0.22199
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	68409	5044
<I/σ(I)>	22.8	2.7
Completeness [%]	99.8	99.8
Redundancy	20	20
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.4 M KCl, 50 mM K/Na Tartrate, 20 mM BaCl2, and 0.1 M Sodium Citrate, pH 5.5