5UN0

Crystal Structure of Mycobacterium Tuberculosis Proteasome-assembly chaperone homologue Rv2125

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	138.250, 79.760, 89.030
Unit cell angles	90.00, 103.57, 90.00

Refinement procedure

Resolution	30.650 - 3.000
R-factor	0.23905
Rwork	0.236
R-free	0.28914
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mnf
RMSD bond length	0.010
RMSD bond angle	1.381
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.650
High resolution limit [Å]	3.000
Number of reflections	18826
<I/σ(I)>	5.7
Completeness [%]	98.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M ammonium sulfate, 1% w/v ANAPOE-20, and 2% w/v benzamidine hydrochloride
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M ammonium sulfate, 1% w/v ANAPOE-20, and 2% w/v benzamidine hydrochloride