5UMW
Crystal structure of TnmS2, an antibiotic binding protein from Streptomyces sp. CB03234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915, 0.97941 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.298, 74.298, 342.783 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.270 |
| R-factor | 0.20845 |
| Rwork | 0.205 |
| R-free | 0.26313 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.958 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.260 | 2.270 |
| Rmerge | 0.077 | 0.085 |
| Rmeas | 0.081 | 0.917 |
| Rpim | 0.027 | 0.335 |
| Number of reflections | 52843 | 48510 |
| <I/σ(I)> | 14.8 | 2.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 9 | 7.3 |
| CC(1/2) | 0.998 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50 mM magnesium sulfate hydrate, 50 mM HEPES sodium, and 1.6 M lithium sulfate monohydrate |
