5UMT

Crystal structure of N-terminal domain of human FACT complex subunit SPT16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.249, 78.068, 111.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.479 - 2.092
R-factor	0.1735
Rwork	0.172
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cb6
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_972)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.480	2.170
High resolution limit [Å]	2.090	2.090
Rmerge	0.093	0.633
Number of reflections	32096	2190
<I/σ(I)>	13.2	8.9
Completeness [%]	99.4	96.1
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	10mM HEPES, 150mM NaCl, 5% glycerol, 20% ethylene glycol