5UMR
Crystal structure of N-terminal domain of human FACT complex subunit SSRP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 43 |
| Unit cell lengths | 37.958, 37.958, 67.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.958 - 1.501 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.18840 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_972) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.450 |
| Number of reflections | 15111 | 2903 |
| <I/σ(I)> | 77.6 | 3 |
| Completeness [%] | 99.0 | 87.5 |
| Redundancy | 12 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 2.2 M DL-Malic acid, 100 mM Bis-Tris propane, pH 7.0. |
