5UMP
Crystal structure of TnmS3, an antibiotic binding protein from Streptomyces sp. CB03234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.630, 67.820, 83.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.080 |
| R-factor | 0.18548 |
| Rwork | 0.185 |
| R-free | 0.20301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hc5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP (5.8.0155) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.049 | 0.620 |
| Rmeas | 0.054 | 0.691 |
| Rpim | 0.021 | 0.298 |
| Number of reflections | 106971 | 4861 |
| <I/σ(I)> | 31.6 | 1.9 |
| Completeness [%] | 95.3 | 88 |
| Redundancy | 6.4 | 4.8 |
| CC(1/2) | 0.997 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM ammonium acetate, 100 mM bis-tris, and 17% w/v polyethylene glycol 3350 |
