5UMN
Crystal structure of C05 VPGSGW mutant bound to H3 influenza hemagglutinin, HA1 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 |
| Unit cell lengths | 88.344, 88.344, 255.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.340 - 1.970 |
| R-factor | 0.18254 |
| Rwork | 0.181 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4FP8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.400 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rpim | 0.066 | 0.353 |
| Number of reflections | 136341 | 13627 |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 6.3 | 6.2 |
| CC(1/2) | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium citrate pH 5.5 and 9% PEG 8000 |
