5ULX

Structure of human DNA polymerase iota bound to template 1-methyl-deoxyadenosine crystallized in the presence of dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97921
Spacegroup name	P 65 2 2
Unit cell lengths	97.915, 97.915, 202.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.960 - 1.960
R-factor	0.217
Rwork	0.216
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fll
RMSD bond length	0.005
RMSD bond angle	0.697
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.960	1.960
Number of reflections	41925
<I/σ(I)>	25.2
Completeness [%]	99.9
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	100 mM MES, pH 6.0, 14 % PEG 5KMME, 0.4 M Ammonium Sulfate