5ULI

Crystal Structure of mouse DXO in complex with (3'-NADP)+ and calcium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.867, 87.678, 53.158
Unit cell angles	90.00, 113.02, 90.00

Refinement procedure

Resolution	48.926 - 2.100
R-factor	0.1649
Rwork	0.161
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.926	48.926	2.230
High resolution limit [Å]	2.100	6.260	2.100
Rmerge	0.072	0.025	0.396
Rmeas	0.088	0.030	0.488
Total number of observations	137567
Number of reflections	47716	1792	7615
<I/σ(I)>	12.06	33.68	2.81
Completeness [%]	98.2	97.9	97.5
Redundancy	2.902	2.972	2.841
CC(1/2)	0.997	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% (w/v) PEG 3350