5ULE

Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	107.143, 102.283, 170.860
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.24705
Rwork	0.247
R-free	0.26554
RMSD bond length	0.006
RMSD bond angle	1.012
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.800
Number of reflections	86060
<I/σ(I)>	26
Completeness [%]	96.0
Redundancy	25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG, Na+, etc.