5UJP
The crystal structure of a glyoxalase/bleomycin resistance protein from Streptomyces sp. CB03234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 31 |
| Unit cell lengths | 55.352, 55.352, 62.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.320 - 1.420 |
| R-factor | 0.1633 |
| Rwork | 0.161 |
| R-free | 0.20210 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.062 | 0.849 |
| Rpim | 0.029 | 0.495 |
| Number of reflections | 40879 | 1984 |
| <I/σ(I)> | 47.1 | 1.2 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 7.2 | 4.7 |
| CC(1/2) | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 297 | 0.2 M Lithium Sulfate, 0.1 M Na2HPO4, 1.2 M NaH2PO4/1.2 M K2HPO4 |
