5UJ8
Human Origin Recognition Complex subunits 2 and 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.260, 114.961, 316.455 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 20.067 - 6.000 |
| R-factor | 0.3205 |
| Rwork | 0.318 |
| R-free | 0.36850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4XGC - chains B C |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.293 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.070 | 6.210 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Number of reflections | 15179 | |
| <I/σ(I)> | 5.7 | 1 |
| Completeness [%] | 95.0 | 99.1 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.990 | 0.422 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 9.5% PEG20000 50mM tri-Sodium-citrate 60mM Citric Acid 10mg/ml HO23 Ratio 1:1 |
