5UIJ
X-ray structure of The FdtF N-formyltransferase from Salmonella enteric O60 in complex with TDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.910, 64.757, 97.757 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 97.750 - 1.900 |
| R-factor | 0.18332 |
| Rwork | 0.181 |
| R-free | 0.23126 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.995 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.750 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.278 |
| Number of reflections | 70013 | 9252 |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 95.6 | 87.2 |
| Redundancy | 3.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS 15-18% PEG 3350 5 mM TDP |
