5UID
The crystal structure of an aminotransferase TlmJ from Streptoalloteichus hindustanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.601, 198.093, 60.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.180 |
| R-factor | 0.1919 |
| Rwork | 0.190 |
| R-free | 0.23280 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.490 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.131 | 0.658 |
| Rpim | 0.056 | 0.293 |
| Number of reflections | 84790 | 4061 |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 95.7 | 94 |
| Redundancy | 6 | 5.7 |
| CC(1/2) | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.8 M Lisium sulfate, 0.1 M Sodium Acetate:HCl |
