5UI3

Crystal structure of DHDPS from chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	110
Detector technology	CCD
Collection date	2015-07-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	71.700, 103.630, 78.130
Unit cell angles	90.00, 95.02, 90.00

Refinement procedure

Resolution	62.230 - 2.000
R-factor	0.2028
Rwork	0.201
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.439
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.230	62.230	2.040
High resolution limit [Å]	2.000	9.800	2.000
Rmerge	0.130	0.048	0.738
Number of reflections	73502
<I/σ(I)>	9.9
Completeness [%]	95.7	98.3	98.6
Redundancy	6.5	6.1	5.6
CC(1/2)	0.995	0.997	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	25% PEG 1500, 10% succinate-phosphate-glycine pH 5