5UHS
Structure of a SemiSWEET D57A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03327 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.021, 36.920, 47.514 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.800 |
| R-factor | 0.2456 |
| Rwork | 0.242 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qnc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.876 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 4006 |
| <I/σ(I)> | 13.73 |
| Completeness [%] | 96.9 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 11.7 MAG 50 mM tris pH 8 100 mM LiSO4 35% PEG 400 40 mM glucose |
