5UHO
Crystal structure of the core catalytic domain of human O-GlcNAcase complexed with PUGNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.340, 97.340, 260.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.380 - 3.210 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.000 | 3.220 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.091 | 1.072 |
| Number of reflections | 21392 | |
| <I/σ(I)> | 22.4 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13.3 |
| CC(1/2) | 0.997 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 14-17% PEG 3350, 0.2 M Mg Formate |
