5UHJ
The crystal structure of a natural product biosynthetic enzyme from Streptomyces sp. CB03234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.836, 33.346, 101.781 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 30.886 - 1.750 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.24320 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.723 |
| Rpim | 0.031 | |
| Number of reflections | 27985 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 4.8 | 3.6 |
| CC(1/2) | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 289 | 0.2 M Magnesium Formate, 20% (w/v) PEG3500 |
