5UH9

Crystal structure of Mycobacterium tuberculosis transcription initiation complex containing 2nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.919
Spacegroup name	P 21 21 21
Unit cell lengths	154.460, 164.627, 201.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.889 - 4.402
R-factor	0.285
Rwork	0.281
R-free	0.33200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.560
High resolution limit [Å]	4.400	9.460	4.400
Rmerge	0.191	0.158	0.542
Rmeas	0.202	0.166	0.624
Rpim	0.062	0.050	0.300
Total number of observations	237561
Number of reflections	31061
<I/σ(I)>	8.6
Completeness [%]	93.3	99.1	81.7
Redundancy	7.6	12.2	3.4
CC(1/2)		0.906	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	50 mM Tris-HCl, pH 8.3, 200 mM KCl, 50 mM MgCl2, and 10% PEG3350