Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127092 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 126.524, 126.524, 103.207 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.440 - 1.560 |
| R-factor | 0.10375 |
| Rwork | 0.102 |
| R-free | 0.13422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l9z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.840 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.440 | 38.440 | 1.590 |
| High resolution limit [Å] | 1.560 | 8.550 | 1.560 |
| Rmerge | 0.060 | 0.031 | 0.522 |
| Number of reflections | 62486 | ||
| <I/σ(I)> | 22.2 | 62 | 2.7 |
| Completeness [%] | 91.1 | 80.2 | 79.3 |
| Redundancy | 11.1 | 11.4 | 5.9 |
| CC(1/2) | 0.999 | 0.998 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M Hepes, pH 7.5, 30 % w/v PEG 400, 0.2 M MgCl2 |
