5UG0

Human antibody H2897 in complex with influenza hemagglutinin H1 Solomon Islands/03/2006

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	293.15
Detector technology	CCD
Collection date	2015-08-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	P 3 2 1
Unit cell lengths	142.200, 142.200, 200.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.410 - 3.400
R-factor	0.242
Rwork	0.240
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3SM5
RMSD bond length	0.009
RMSD bond angle	1.260
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.410	46.410	3.610
High resolution limit [Å]	3.400	10.000	3.400
Rmerge	0.274	0.024	3.473
Rmeas	0.322
Total number of observations	119057
Number of reflections	32426
<I/σ(I)>	5.75	36.01	0.44
Completeness [%]	98.6	95	97
Redundancy	3.672	3.294	3.726
CC(1/2)	0.987	0.998	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% w/v PEG8000, 100 mM HEPES