5UFD
Crystal Structure of Variable Lymphocyte Receptor (VLR) RBC36 (Apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03316 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.339, 78.895, 158.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.957 - 1.696 |
| R-factor | 0.1862 |
| Rwork | 0.185 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6j |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.957 | 1.760 |
| High resolution limit [Å] | 1.696 | 1.696 |
| Rpim | 0.020 | 0.160 |
| Number of reflections | 60711 | |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 17.3 mg/mL RBC36, 100 mM Tris, pH 8.5, 200 mM magnesium chloride, 20% PEG8000 |
