5UF5

Structure of the effector protein SidK (lpg0968) from Legionella pneumophila (domain-swapped dimer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-08
Detector	RAYONIX MX-300
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	162.080, 41.140, 94.150
Unit cell angles	90.00, 108.39, 90.00

Refinement procedure

Resolution	45.336 - 2.400
R-factor	0.1972
Rwork	0.195
R-free	0.22960
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.454
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.340	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.125	1.050
Number of reflections	23542
<I/σ(I)>	13.4	1.98
Completeness [%]	99.9	99.8
Redundancy	6.2	6.3
CC(1/2)	0.998	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M sodium cacodylate trihydrate pH 6.5, 0.2 M magnesium acetate tetrahydrate, 20% (w/v) polyethylene glycol 8,000