5UF2
Crystal Structure of ribose 5 phosphate isomerase A from Neisseria gonorrhoeae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-10 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.650, 75.870, 46.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.400 |
R-factor | 0.1363 |
Rwork | 0.135 |
R-free | 0.16350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m0s |
RMSD bond length | 0.005 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.440 |
High resolution limit [Å] | 1.400 | 6.260 | 1.400 |
Rmerge | 0.075 | 0.045 | 0.510 |
Number of reflections | 49825 | ||
<I/σ(I)> | 14.67 | 33.41 | 3.24 |
Completeness [%] | 99.0 | 85.6 | 100 |
Redundancy | 5.69 | 5.697 | 5.626 |
CC(1/2) | 0.998 | 0.997 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MCSG1 F1 (283224f1): 100mM Bis-Tris:HCl pH6.5, 20% (w/v) PEG 5000 MME: protein conc. 18.44mg/mL, cryoprotectant 20% ethylene glycol, puck kgc9-3 |