5UEV

BRD4_BD2_A-556343

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	56.426, 73.514, 33.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.760 - 1.940
R-factor	0.201
Rwork	0.200
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo BRD4_BD2
RMSD bond length	0.010
RMSD bond angle	0.920
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	73.500
High resolution limit [Å]	1.930
Number of reflections	10978
<I/σ(I)>	9.5
Completeness [%]	99.8
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0