5UEU

BRD4_BD2_A-1107604

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-07-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	55.826, 64.544, 75.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.220 - 2.260
R-factor	0.189
Rwork	0.187
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo BRD4_BD2
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	75.300
High resolution limit [Å]	2.260
Number of reflections	13221
<I/σ(I)>	14.4
Completeness [%]	100.0
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0