5UET

BRD4_BD2_A-1308586

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	59.123, 72.965, 33.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.480 - 2.290
R-factor	0.214
Rwork	0.212
R-free	0.25400
RMSD bond length	0.008
RMSD bond angle	0.860
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	73.000
High resolution limit [Å]	2.270
Number of reflections	7155
<I/σ(I)>	16.8
Completeness [%]	99.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0