5UER

BRD4_BD2_A-1359930

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-08-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	56.690, 72.107, 33.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.620 - 1.870
R-factor	0.202
Rwork	0.200
R-free	0.24300
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	72.100
High resolution limit [Å]	1.870
Number of reflections	11581
<I/σ(I)>	25.6
Completeness [%]	97.1
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0