5UEP

BRD4_BD2_A-581577

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	56.802, 73.604, 33.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.800 - 1.770
R-factor	0.184
Rwork	0.182
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo BRD4_BD2
RMSD bond length	0.010
RMSD bond angle	0.900
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	73.600
High resolution limit [Å]	1.770
Number of reflections	14367
<I/σ(I)>	14.8
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris 7.0