5UEF

RNA primer-template complex with guanosine dinucleotide p(5')G(3')p(5')G ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2016-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.997
Spacegroup name	P 3 2 1
Unit cell lengths	43.628, 43.628, 84.051
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.24289
Rwork	0.240
R-free	0.30318
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.019
RMSD bond angle	2.062
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.074	0.469
Number of reflections	5804
<I/σ(I)>	30.86	2.78
Completeness [%]	99.8	99.6
Redundancy	9.6	7
CC(1/2)	0.979	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.05 M Magnesium chloride, 0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M Ammonium sulfate