5UE7
Crystal structure of the phosphomannomutase PMM1 from Candida albicans, apoenzyme state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 62.225, 80.719, 116.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.696 - 1.950 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2amy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.722 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.063 | 0.806 |
| Number of reflections | 43607 | |
| <I/σ(I)> | 36.97 | 2.75 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.7 | 5.6 |
| CC(1/2) | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 20% PEG3350, 0.2 M MgCl2, 0.1 M Bis-tris pH 5.5, 1% DMSO |
