5UE7

Crystal structure of the phosphomannomutase PMM1 from Candida albicans, apoenzyme state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	62.225, 80.719, 116.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.696 - 1.950
R-factor	0.2104
Rwork	0.208
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2amy
RMSD bond length	0.005
RMSD bond angle	0.722
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.063	0.806
Number of reflections	43607
<I/σ(I)>	36.97	2.75
Completeness [%]	99.5	99.9
Redundancy	5.7	5.6
CC(1/2)		0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	20% PEG3350, 0.2 M MgCl2, 0.1 M Bis-tris pH 5.5, 1% DMSO