5UE0
1.90 A resolution structure of CT622 C-terminal domain from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 121.812, 121.812, 121.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.556 - 1.900 |
| R-factor | 0.2132 |
| Rwork | 0.211 |
| R-free | 0.25790 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.065 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2502: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.910 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 23829 | |
| <I/σ(I)> | 22.6 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.2 |
| CC(1/2) | 0.999 | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Bis-Tris (pH 7.2), 1.9 M Ammonium Sulfate |
