5UDO
Crystal structure of the coiled-coil domain from Listeria Innocua Phage Integrase (Tetragonal Form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-03 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | .976 |
| Spacegroup name | P 41 |
| Unit cell lengths | 98.017, 98.017, 52.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.853 - 2.541 |
| R-factor | 0.2554 |
| Rwork | 0.252 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kis |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.423 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.541 | 2.541 |
| Rmerge | 0.091 | 0.615 |
| Number of reflections | 16440 | |
| <I/σ(I)> | 12.9 | 1.95 |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.16 | 0.8-1.2 M Na Citrate |
