5UDI

IFIT1 monomeric mutant (L457E/L464E) with m7Gppp-AAAA (syn and anti conformations of cap)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	94
Detector technology	CCD
Collection date	2015-12-02
Detector	RAYONIX MX300HE
Wavelength(s)	0.979
Spacegroup name	P 42 2 2
Unit cell lengths	111.715, 111.715, 93.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.123 - 1.580
R-factor	0.1584
Rwork	0.157
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.579
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2142)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.579	4.290	1.580
Rmerge	0.078	0.039
Number of reflections	81091
<I/σ(I)>	33.6		1.74
Completeness [%]	100.0	99.8	100
Redundancy	14.5	13.9	12.4
CC(1/2)		0.999	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	277.15	27 - 32 % PEG 200, 0.1 M Tris pH 8.1, 200 mM CaCl2