5UDE
Crystal Structure of RSV F B9320 DS-Cav1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 170.430, 170.430, 170.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.608 - 3.001 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mms |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.610 | 42.610 | 3.210 |
| High resolution limit [Å] | 3.000 | 8.490 | 3.000 |
| Rmerge | 0.209 | 0.050 | 0.972 |
| Rmeas | 0.229 | ||
| Rpim | 0.093 | ||
| Total number of observations | 99952 | ||
| Number of reflections | 17606 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.5 | 95.7 | 100 |
| Redundancy | 5.7 | 5.1 | 5.6 |
| CC(1/2) | 0.988 | 0.997 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% w/v PEG1500, 0.1 M Tris pH 7.5, 0.1 M ammonium sulfate |
