5UD1
Class II fructose-1,6-bisphosphate aldolase H180Q variant of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.03322 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.301, 64.207, 62.520 |
| Unit cell angles | 81.37, 74.10, 75.94 |
Refinement procedure
| Resolution | 28.608 - 1.795 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.608 |
| High resolution limit [Å] | 1.795 |
| Rmerge | 0.069 |
| Number of reflections | 49683 |
| <I/σ(I)> | 7.7 |
| Completeness [%] | 84.3 |
| Redundancy | 1.8 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer |
