5UCZ
Class II fructose-1,6-bisphosphate aldolase E149A variant of Helicobacter pylori with DHAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.474, 86.133, 91.196 |
Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
Resolution | 29.911 - 1.780 |
R-factor | 0.1864 |
Rwork | 0.184 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4u |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2481)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.911 |
High resolution limit [Å] | 1.780 |
Rmerge | 0.085 |
Number of reflections | 54638 |
<I/σ(I)> | 10.86 |
Completeness [%] | 87.3 |
Redundancy | 3.3 |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer |